研究業績

2022年

本研究プログラムの成果

• Ayaka Tatsukawa, Yu Tanaka, Haruka Nagano, Atsushi Fukumoto, Yojiro Anzai and Kenji Arakawa*.
  Isolation, biosynthetic investigation, and biological evaluation of maniwamycin G, an azoxyalkene compound from Streptomyces sp. TOHO-M025.
  Journal of Natural Products, 85 [7], 1867-1871. doi: 10.1021/acs.jnatprod.2c00131. (2022)

• Xiaofei Shao, Chang Zheng, Peng Xu, Taro Shiraishi, Tomohisa Kuzuyama, Antonio Molinaro, Alba Silipo and Biao Yu.
  Total Synthesis and Stereochemistry Assignment of Nucleoside Antibiotic A-94964.
  Angew. Chem. Int. Ed. Engl., 61(14):e202200818, doi: 10.1002/anie.202200818. (2022)

• Ryuhei Nagata, Hironori Suemune, Masaya Kobayashi, Tetsuro Shinada, Kazuo Shin-ya, Makoto Nishiyama, Tomoya Hino, Yusuke Sato, Tomohisa Kuzuyama and Shingo Nagano.
  Structural Basis for the Prenylation Reaction of Carbazole-Containing Natural Products Catalyzed by Squalene Synthase-Like Enzymes.
  Angew. Chem Int. Ed. Engl., 61(2):e202117430. doi: 10.1002/anie.202117430. (2022)

• Yuya Misaki, Yosi Nindita, Kota Fujita, Amirudin Akhmad Fauzi and Kenji Arakawa.
  Overexpression of SRO_3163, a homolog of Streptomyces antibiotic regulatory protein, induces the production of novel cyclohexene-containing enamide in Streptomyces rochei.
  Biosci. Biotechnol. Biochem., 86 [2], 177-184. doi: 10.1093/bbb/zbab206. (2022)

• Rukman Muslimin, Natsumi Nishiura, Aiko Teshima, Kiep Minh Do, Takeshi Kodama, Hiroyuki Morita, Cody Wayne Lewis, Gordon Chan, Ahmed Taha Ayoub and Kenji Arakawa.
  Chemoenzymatic synthesis, computational investigation, and antitumor activity of monocyclic lankacidin derivatives.
  Bioorganic & Medicinal Chemistry, 53, 116551. doi: 10.1016/j.bmc.2021.116551. (2022)

• Yuya Misaki, Yuzuru Takahashi, Keisuke Hara, Satoshi Tatsuno and Kenji Arakawa.
  Three 4-monosubstituted butyrolactones from a regulatory gene mutant of Streptomyces rochei 7434AN4.
  J. Biosci. Bioeng., 133 [4], 329-334. doi: 10.1016/j.jbiosc.2022.01.006. (2022)

• Seiji Kawai, Yohei Katsuyama and Yasuo Ohnishi.
  The α/β hydrolase AzpM catalyzes dipeptide synthesis in alazopeptin biosynthesis using two Molecules of carrier protein-tethered amino acid.
  Chembiochem, e202100700. doi:10.1002/cbic.202100700. (2022)

• Tezuka Takeaki and Yasuo Ohnishi.
  Surface structure and nanomechanical properties of Actinoplanes missouriensis sporangia analyzed via atomic force microscopy.
  Biosci. Biotech. Biochem., 86(4), 552-556. doi: 10.1093/bbb/zbac002. (2022)

• Yuki Ogawa, Yohei Katsuyama and Yasuo Ohnishi.
  Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning.
  ACS Synth. Biol., 2022, 11, 1, 473–485, doi: 10.1021/acssynbio.1c00564. (2022)

• Kazuya Yamanaka, Hibiki Fukumoto, Naoki Yoshimura, Kenji Arakawa, Yasuo Kato and Yoshimitsu Hamano.
  Discovery of a Polyamino Acid Antibiotic Solely Comprising L-beta-Lysine by Potential Producer Prioritization-Guided Genome Mining.
  ACS Chem. Biol., 17, 171-180. doi: 10.1021/acschembio.1c00832. (2022)

その他の成果

• Ayumi Hikima, Shumpei Asamizu, Hiroyasu Onaka, Huiping Zhang, Hiroshi Tomoda and Nobuhiro Koyama.
  Kimidinomycin, a new antibiotic against Mycobacterium avium complex, produced by Streptomyces sp. KKTA-0263.
  J Antibiot (Tokyo). 75(2), 72-76, doi: 10.1038/s41429-021-00494-3. (2022)

• Yoshiki Asai, Tomoshige Hiratsuka, Miyu Ueda, Yumi Kawamura, Shumpei Asamizu, Hiroyasu Onaka, Manabu Arioka, Shinichi Nishimura and Minoru Yoshida.
  Differential Biosynthesis and Roles of Two Ferrichrome-Type Siderophores, ASP2397/AS2488053 and Ferricrocin, in Acremonium persicinum.
  ACS Chem Biol. 17(1), 207-216, doi: 10.1021/acschembio.1c00867. (2022)

• Aiko Tanaka, Maarten H. Ryder, Takamasa Suzuki, Kazuma Uesaka, Nobuo Yamaguchi, Tomoko Amimoto, Motoyasu Otani, Osamu Nakayachi, Kenji Arakawa, Nobukazu Tanaka, Daigo Takemoto.
  Production of agrocinopine A by Ipomoea batatas agrocinopine synthase in transgenic tobacco and its effect on the rhizosphere microbial community.
  Molecular Plant-Microbe Interactions, 35 [1], 73-84. doi: 10.1094/MPMI-05-21-0114-R. (2022)

• Kazuya Yamanaka, Hibiki Fukumoto, Naoki Yoshimura, Kenji Arakawa, Yasuo Kato, Yoshimitsu Hamano and Tadao Oikawa.
  Discovery of a poly-amino acid antibiotic solely comprising of L-β-lysine by potential producer prioritization-guided genome mining.
  ACS Chemical Biology, 17 [1], 171-180. doi:10.1021/acschembio.1c00832. (2022)

• Tomohiro Noguchi, Shota Isogai, Tohru Terada, Makoto Nishiyama and Tomohisa Kuzuyama*.
  Cryptic Oxidative Transamination of Hydroxynaphthoquinone in Natural Product Biosynthesis.
  J Am Chem Soc., 144(12):5435-5440. doi: 10.1021/jacs.1c13074. (2022)

• Taichi Chisuga, Akira Nagai, Akimasa Miyanaga, Ena Goto, Kosuke Kishikawa, Fumitaka Kudo and Tadashi Eguchi.
  Structural Insight into the Reaction Mechanism of Ketosynthase-like Decarboxylase in a Loading Module of Modular Polyketide Synthases.
  ACS Chem. Biol., 17(1), 198-206, doi: 10.1021/acschembio.1c00856. (2022)

• Takaki Okamoto, Kazuya Yamanaka, Yoshimitsu Hamano, Shingo Nagano and Tomoya Hino.
  Crystal structure of the adenylation domain from an ε-poly-l-lysine synthetase provides molecular mechanism for substrate specificity.
  Biochem. Biophys. Res. Commun., 596, 43-48 (2022).

• Naoya Oku, Shion Takemura, Hiroyasu Onaka* and Yasuhiro Igarashi*.
  NMR characterization of streptogramin B and L-156,587, a non-synergistic pair of the streptogramin family antibiotic complexes produced inductively by a combined culture of Streptomyces albogriseolus and Tsukamurella pulmonis.
  MRC, 60(2): 261-270. doi: 10.1002/mrc.5219. (2022)

2021年

本研究プログラムの成果

• Kazuya Yamanaka, Ryo Ozaki, Yoshimitsu Hamano and Tadao Oikawa.
  Molecular and Mechanistic Characterization of PddB, the First PLP-independent 2,4-diaminobutyric Acid Racemase Discovered in an Actinobacterial D-amino Acid Homopolymer Biosynthesis.
  Front. Microbiol., 12, 686023. doi: 10.3389/fmicb.2021.686023. (2021)

• Shiraishi T, Xia J, Kato T and Kuzuyama T.
  Biosynthesis of the nucleoside antibiotic angustmycins: identification and characterization of the biosynthetic gene cluster reveal unprecedented dehydratase required for exo-glycal formation.
  J Antibiot, 2021 Nov;74(11):830-833. doi: 10.1038/s41429-021-00466-7. (2021)

• Seiji Kawai, Yuko Sugaya, Ryota Hagihara, Hiroya Tomita, Yohei Katsuyama and Yasuo Ohnishi.
  Complete Biosynthetic Pathway of Alazopeptin, a Tripeptide Consisting of Two Molecules of 6‐Diazo‐5‐oxo‐l‐norleucine and One Molecule of Alanine.
  Angew. Chem. Int. Ed., 60(18), 10319-10325, doi: 10.1002/anie.202100462. (2021)

• Fumitaka Kudo, Sotaro Takahashi, Akimasa Miyanaga, Yuichiro Nakazawa, Kota Nishino, Yuki Hayakawa, Koichi Kawamura, Fumihiro Ishikawa, Genzoh Tanabe, Naeko Iwai, Yoko Nagumo, Takeo Usui and Tadashi Eguchi.
  Mutational biosynthesis of hitachimycin analogs controlled by the β-amino acid-selective adenylation enzyme HitB.
  ACS Chem. Biol., 16, 3, 539–547, doi: 10.1021/acschembio.1c00003. (2021)

• Aiko Teshima, Hisashi Kondo, Yu Tanaka, Yosi Nindita, Yuya Misaki, Yuji Konaka, Yasuhiro Itakura, Tsugumi Tonokawa, Haruyasu Kinashi and Kenji Arakawa.
  Substrate specificity of two cytochrome P450 monooxygenases involved in lankamycin biosynthesis.
  Biosci. Biotechnol. Biochem., 85, 115-125, doi: 10.1093/bbb/zbaa063. (2021)

• Taro Shiraishi and Tomohisa Kuzuyama.
  Biosynthetic pathways and enzymes involved in the production of phosphonic acid natural products.
  Biosci. Biotechnol. Biochem., 85(1), 42-52, doi: 10.1093/bbb/zbaa052. (2021)

• Hayama Tsutsumi, Yoshitaka Moriwaki, Tohru Terada, Kentaro Shimizu, Kazuo Shin-Ya, Yohei Katsuyama and Yasuo Ohnishi.
  Structural and molecular basis of the catalytic mechanism of geranyl pyrophosphate C6-methyltransferase: creation of an unprecedented farnesyl pyrophosphate C6-methyltransferase.
  Angew. Chem. Int. Ed. Engl., 61(1), e202111217. doi: 10.1002/anie.202111217. Epub 2021 Nov 23.(2021)

• Hiroya Tomita, Yohei Katsuyama and Yasuo Ohnishi.
  Rapid evaluation of the substrate specificity of 3-nitrobenzoic acid dioxygenase MnbAB via colorimetric detection using Saltzman reagent.
  J. Ind. Microbiol. Biotechnol., 48, kuab064. (2021)

• Yohei Katsuyama, Kaoru Sone, Ayaka Harada, Seiji Kawai, Naoki Urano, Naruhiko Adachi, Toshio Moriya, Masato Kawasaki, Kazuo Shin-ya, Toshiya Senda and Yasuo Ohnishi.
  Structural and functional analyses of the tridomain‐nonribosomal peptide synthetase FmoA3 for 4‐methyloxazoline ring formation.
  Angew. Chem. Int. Ed., 2021, 5, 2000193., doi.org/10.1002/anie.202102760. (2021)

その他の成果

• Shusuke Sato, Fumitaka Kudo, Michel Rohmer, Tadashi Eguchi.
  Biochemical and Mutational Analysis of Radical S-Adenosyl-L-methionine adenosylhopane Synthase HpnH from Zymomonas mobilis Reveals that the Conserved Residue Cysteine-106 Reduces a Radical Intermediate and Determines the Stereochemistry.
  Biochemistry, 60(38), 2865-2874, doi: 10.1021/acs.biochem.1c00536. (2021)

• Akimasa Miyanaga, Risako Ouchi, Fumitaka Kudo, Tadashi Eguchi.
  Complex Structure of the Acyltransferase VinK and the Carrier Protein VinL with a Pantetheine Cross-linking Probe.
  Acta Crystallographica Section F, 77(9), 294-302, doi:10.1107/S2053230X21008761. (2021)

• T. Nagakubo, T. Yamamoto, S. Asamizu, M. Toyofuku, N. Nomura, H. Onaka*.
  Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi.
  Scientific Reports, 11(1), 20116. doi:10.1038/s41598-021-99490-8. (2021)

• Fumitaka Kudo, Yukinobu Kitayama, Akimasa Miyanaga, Mario Numakura and Tadashi Eguchi.
  Stepwise post-glycosylation modification of sugar moieties in kanamycin biosynthesis.
  ChemBioChem, 22(9), 1668-1675, doi: 10.1002/cbic.202000839 (2021)

• Tomohiro Suzuki, Takeo Tomita, Kenta Hirayama, Michio Suzuki, Tomohisa Kuzuyama and Makoto Nishiyama.
  Involvement of subdomain II in the recognition of acetyl-CoA revealed by the crystal structure of homocitrate synthase from Sulfolobus acidocaldarius.
  FEBS J., 288(6), 1975-1988, doi: 10.1111/febs.15527. (2021)

• Sivamoke Dissook, Tomohisa Kuzuyama, Yuri Nishimoto, Shigeru Kitani, Sastia Putri and Eiichiro Fukusaki.
  Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica.
  Sci Rep., 11(1), 5598, doi: 10.1038/s41598-021-85170-0. (2021)

• Jie Zhang, Satoshi Yuzawa, Wei Li Thong, Tetsuro Shinada, Makoto Nishiyama and Tomohisa Kuzuyama.
  Reconstitution of a Highly Reducing Type II PKS System Reveals 6π-Electrocyclization Is Required for o-Dialkylbenzene Biosynthesis.
  J. Am. Chem. Soc., 143(7), 2962-2969, doi: 10.1021/jacs.0c13378. (2021)

• Takuya Hashimoto, Junko Hashimoto, Noritaka Kagaya, Takehiro Nishimura, Hikaru Suenaga, Makoto Nishiyama, Tomohisa Kuzuyama and Kazuo Shin-ya.
  A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster.
  J. Antibiot., 74, 354-358, doi: 10.1038/s41429-021-00410-9. (2021)

• Mitsuki Seo, Danyao Du, Yohei Katsuyama, Ryo Katsuta, Arata Yajima, Tomoo Nukada, Yasuo Ohnishi and Ken Ishigami.
  Enantioselective synthesis and stereochemical determination of the highly reduced polyketide ishigamide.
  Biosci. Biotechnol. Biochem., 85, 148–153, doi: 10.1093/bbb/zbaa023. (2021)

• Fumitaka Kudo, Ayaka Mori, Mai Koide, Ryo Yajima, Ryohei Takeishi, Akimasa Miyanaga and Tadashi Eguchi.
  One-pot enzymatic synthesis of 2-deoxy-scyllo-inosose from D-glucose and polyphosphate.
  Biosci. Biotechnol. Biochem., 85(1), 108-114, doi: 10.1093/bbb/zbaa025. (2021)

2020年

本研究プログラムの成果

• Kazuya Yamanaka, Hibiki Fukumoto, Munenori Takehara, Yoshimitsu Hamano, and Tadao Oikawa.
  The Stereocontrolled Biosynthesis of Mirror-symmetric 2,4-diaminobutyric Acid Homopolymers is Critically Governed by Adenylation Activations
  ACS Chem. Biol., 7, 1967-1973. doi: 10.1021/acschembio.0c00321. (2020)

• Chitose Maruyama and Yoshimitsu Hamano.
  tRNA-dependent amide bond–forming enzymes in peptide natural product biosynthesis.
  Curr. Opin. Chem. Biol., 59, 164-171, doi: 10.1016/j.cbpa.2020.08.002. (2020)

• Masaya Kobayashi and Tomohisa Kuzuyama.
  Recent Advances in the Biosynthesis of Carbazoles Produced by Actinomycetes.
  Biomolecules, 10(8), 1147, doi: 10.3390/biom10081147. (2020)

• Aiko Teshima, Nozomi Hadae, Naoto Tsuda and Kenji Arakawa.
  Functional analysis of P450 monooxygenase SrrO in the biosynthesis of butenolide-type signaling molecules in Streptomyces rochei.
  Biomolecules, 10(9), 1237, doi: 10.3390/biom10091237. (2020)

• Yuichiro Hashiguchi, Takeaki Tezuka, Yoshihiro Mouri, Kenji Konishi, Azusa Fujita, Aiko Hirata and Yasuo Ohnishi.
  Regulation of sporangium formation, spore dormancy, and sporangium dehiscence by a hybrid sensor histidine kinase in Actinoplanes missouriensis: relationship with the global transcriptional regulator TcrA.
  J. Bacteriol., 202(21), e00228-20, doi: 10.1128/JB.00228-20. (2020)

• Chitose Maruyama, Yukiko Chinone, Shusuke Sato, Fumitaka Kudo, Kosuke Ohsawa, Junya Kubota, Junko Hashimoto, Ikuko Kozone, Takayuki Doi, Kazuo Shin-Ya, Tadashi Eguchi and Yoshimitsu Hamano.
  C-Methylation of S-adenosyl-L-methionine occurs prior to cyclopropanation in the biosynthesis of 1-amino-2-methylcyclopropanecarboxylic acid (norcoronamic acid) in a bacterium.
  Biomolecules, 10(5), doi: 10.3390/biom10050775. (2020)

• Yuya Misaki, Shouji Yamamoto, Toshihiro Suzuki, Miyuki Iwakuni, Hiroaki Sasaki, Yuzuru Takahashi, Kuninobu Inada, Haruyasu Kinashi and Kenji Arakawa.
  SrrB, a pseudo-receptor protein, acts as a negative regulator for lankacidin and lankamycin production in Streptomyces rochei.
  Frontiers in Microbiology, 11, 1089, doi: 10.3389/fmicb.2020.01089. (2020)

• Danyao Du, Yohei Katsuyama, Masanobu Horiuchi, Shinya Fushinobu, Aochiu Chen, Tony D. Davis, Michael D. Burkart and Yasuo Ohnishi.
  Structural basis for selectivity in a highly reducing type II polyketide synthase.
  Nat. Chem. Biol., 16, 776–782, doi: 10.1038/s41589-020-0530-0. (2020)

• Ryosuke Kozakai, Takuto Ono, Shotaro Hoshino, Hidenori Takahashi, Yohei Katsuyama, Yoshinori Sugai, Taro Ozaki, Kazuya Teramoto, Kanae Teramoto, Koichi Tanaka, Ikuro Abe, Shumpei Asamizu and Hiroyasu Onaka.
  Acyltransferase that catalyses the condensation of polyketide and peptide moieties of goadvionin hybrid lipopeptides.
  Nature Chemistry, 12, 869–877, doi:10.1038/s41557-020-0508-2. (2020)

• Alexander A. Vinogradov, Morito Shimomura, Yuki Goto, Taro Ozaki, Shumpei Asamizu, Yoshinori Sugai, Hiroaki Suga and Hiroyasu Onaka.
  Minimal lactazole scaffold for in vitro thiopeptide bioengineering.
  Nat. Commun., 11, 2272, doi:10.1038/s41467-020-16145-4. (2020)

その他の成果

• A. A. Vinogradov*, E. Nagai, J. S. Chang, K. Narumi, H. Onaka*, Y. Goto*, H. Suga*.
  Accurate Broadcasting of Substrate Fitness for Lactazole Biosynthetic Pathway from Reactivity-Profiling mRNA Display.
  Journal of the American Chemical Society, 142, 48, 20329-20334. doi:10.1021/jacs.0c10374. (2020)

• Aniruddha Nag, Mohammad Asif Ali, Hideo Kawaguchi, Shun Saito, Yukie Kawasaki, Shoko Miyazaki, Hirotoshi Kawamoto, Deddy Triyono Nugroho Adi, Kumiko Yoshihara, Shunsuke Masuo, Yohei Katsuyama, Akihiko Kondo, Chiaki Ogino, Naoki Takaya, Tatsuo Kaneko and Yasuo Ohnishi.
  Ultrahigh Thermoresistant Lightweight Bioplastics Developed from Fermentation Products of Cellulosic Feedstock.
  Adv. Sustainable Syst., 2021, 5, 2000193. (2020)

• Eiichiro Kan, Hiroya Tomita, Yohei Katsuyama, Jun-Ichi Maruyama, Yasuji Koyama and Yasuo Ohnishi.
  Discovery of the 2,4′-dihydroxy-3′-methoxypropiophenone biosynthesis genes in Aspergillus oryzae.
  Chembiochem, 5, 22(1), 203-211, doi: 10.1002/cbic.202000505. (2020)

• Akimasa Miyanaga, Shohei Kurihara, Taichi Chisuga, Fumitaka Kudo, Tadashi Eguchi.
  Structural characterization of complex of adenylation domain and carrier protein by using pantetheine cross-linking probe.
  ACS Chem. Biol., 15(7), 1808-1812, doi: 10.1021/acschembio.0c00403. (2020)

• Sumire Kurosawa, Kenichi Matsuda, Fumihito Hasebe, Taro Shiraishi, Kazuo Shin-Ya, Tomohisa Kuzuyama and Makoto Nishiyama.
  Guanidyl modification of the 1-azabicyclo[3.1.0]hexane ring in ficellomycin essential for its biological activity.
  Org. Biomol. Chem., 18(27), 5137-5144, doi: 10.1039/d0ob00339e. (2020)

• Akimasa Miyanaga, Ryoma Takaku, Makoto Takaishi, Etsu Tashiro, Fumitaka Kudo and Tadashi Eguchi.
  Generation of incednine derivatives by mutasynthesis.
  J. Antibiot., 73(11), 794-797, doi: 10.1038/s41429-020-0329-y. (2020)

• Yohei Katsuyama and Matsuda Kenichi.
  Recent advance in the biosynthesis of nitrogen–nitrogen bond–containing natural products.
  Curr. Opin. Chem. Biol., 59, 62-68. doi: 10.1016/j.cbpa.2020.05.002. (2020)

• Fumitaka Kudo, Yukinobu Kitayama, Akimasa Miyanaga, Akane Hirayama and Tadashi Eguchi.
  Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis.
  Biochemistry, 59(15), 1470-1473, doi: 10.1021/acs.biochem.0c00204. (2020)

• Yuichiro Hashiguchi, Takeaki Tezuka and Yasuo Ohnishi.
  Involvement of three FliA-family sigma factors in the sporangium formation, spore dormancy and sporangium dehiscence in Actinoplanes missouriensis.
  Mol. Microbiol., 113(6), 1170-1188, doi:10.1111/mmi.14485. (2020)

• Kei Sakaki, Keita Ohishi, Tetsu Shimizu, Ikki Kobayashi, Naoki Mori, Kenichi Matsuda, Takeo Tomita, Hidenori Watanabe, Kan Tanaka, Tomohisa Kuzuyama and Makoto Nishiyama.
  A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria.
  Nat. Chem. Biol., 16(4), 415-422, doi: 10.1038/s41589-019-0461-9. (2020)

• Shun Saito, Wataru Kato, Hiroaki Ikeda, Yohei Katsuyama, Yasuo Ohnishi and Masaya Imoto.
  Discovery of “heat shock metabolites” produced by thermotolerant actinomycetes in high-temperature culture.
  J. Antibiot., 73, 203–21, doi: 10.1038/s41429-020-0279-4. (2020)

2019年

本研究プログラムの成果

• Taro Shiraishi and Tomohisa Kuzuyama.
  Recent advances in the biosynthesis of nucleoside antibiotics.
  J. Antibiot., 72(12), 913-923, doi: 10.1038/s41429-019-0236-2. (2019)

• Taro Ozaki, Ryousuke Sugiyama, Morito Shimomura, Shinichi Nishimura, Asamizu Shumpei, Katsuyama Yohei, Kakeya Hideaki and Onaka Hiroyasu.
  Identification of the common biosynthetic gene cluster for both antimicrobial streptoaminals and antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines.
  Org. Biomol. Chem., 17, 2370-2378, doi: 10.1039/c8ob02846j. (2019)

• Daisuke Kawasaki, Akimasa Miyanaga, Taichi Chisuga, Fumitaka Kudo and Tadashi Eguchi.
  Functional and structural analyses of split-DH domain in the biosynthesis of macrolactam polyketide cremimycin.
  Biochemistry, 58(48), 4799-4803, doi: 10.1021/acs.biochem.9b00897. (2019)

• Keiichi Murai, Lukas Lauterbach, Kazuya Teramoto, Zhiyang Quan, Lena Barra, Tsuyoshi Yamamoto, Kenichi Nonaka, Kazuro Shiomi, Makoto Nishiyama, Tomohisa Kuzuyama and Jeroen S. Dickschat.
  An Unusual Skeletal Rearrangement in the Biosynthesis of the Sesquiterpene Trichobrasilenol from Trichoderma.
  Angew. Chem. Int. Ed., 58(42), 15046-15050, doi: 10.1002/anie.201907964. (2019)

• Yuki Ogawa, Yohei Katsuyama, Kento Ueno and Yasuo Ohnishi.
  Switching the ligand specificity of the biosensor XylS from meta to para-toluic acid through directed evolution exploiting a dual selection system.
  ACS Synth. Biol., 8(12), 2679-2689. doi: 10.1021/acssynbio.9b00237. (2019)

• Masaya Kobayashi, Takeo Tomita, Kazuo Shin‐ya, Makoto Nishiyama and Tomohisa Kuzuyama.
  An Unprecedented Cyclization Mechanism in the Biosynthesis of Carbazole Alkaloids in Streptomyces.
  Angew. Chem. Int. Ed., 58(38), 13349-13353, doi: 10.1002/anie.201906864 (2019)

• Yohei Katsuyama.
  Biosynthesis of aminobenzoic acid derived secondary metabolites from actinobacteria.
  Actinomycetologica, 33(1), S65-S72. (2019)

• Daisuke Kawasaki, Taichi Chisuga, Akimasa Miyanaga, Fumitaka Kudo and Tadashi Eguchi.
  Structural analysis of the glycine oxidase homolog CmiS2 reveals a unique substrate recognition mechanism for formation of a β-amino acid starter unit in cremimycin biosynthesis.
  Biochemistry, 58(24), 2706-2709, doi: 10.1021/acs.biochem.9b00444. (2019)

• Yosi Nindita, Zhisheng Cao, Amirudin Akhmad Fauzi, Aiko Teshima, Yuya Misaki, Rukman Muslimin, Yingjie Yang, Yuh Shiwa, Hirofumi Yoshikawa, Michihira Tagami, Alexander Lezhava, Jun Ishikawa, Makoto Kuroda, Tsuyoshi Sekizuka, Kuninobu Inada, Haruyasu Kinashi and Kenji Arakawa.
  The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids.
  Scientific Reports, 9, 10973, doi: 10.1038/s41598-019-47406-y. (2019)

• Anthony J. Romo, Taro Shiraishi, Hideo Ikeuchi, Geng-Min Lin, Yujie Geng, Yu-Hsuan Lee, Priscilla H. Liem, Tianlu Ma, Yasushi Ogasawara, Kazuo Shin-ya, Makoto Nishiyama, Tomohisa Kuzuyama* and Hung-wen Liu*.
  The Amipurimycin and Miharamycin Biosynthetic Gene Clusters: Unraveling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics.
  J. Am. Chem. Soc., 141(26), 14152-14159, doi: 10.1021/jacs.9b03021. (2019)

• Yohei Katsuyama.
  Mining novel biosynthetic machineries of secondary metabolites from actinobacteria.
  Biosci. Biotechnol. Biochem., 83(9), 1606-1615, doi: 10.1080/09168451.2019.1606700. (2019)

• Kazuya Yamanaka and Yoshimitsu Hamano.
  Draft genome sequence of the most traditional ε-poly-L-lysine producer, Streptomyces albulus NBRC14147.
  Microbiol. Resour. Announc., 8, e01515-18 , doi: 10.1128/MRA.01515-18. (2019)

• Taro Shiraishi, Makoto Nishiyama and Tomohisa Kuzuyama.
  Biosynthesis of the Uridine-Derived Nucleoside Antibiotic A-94964: Identification and Characterization of the Biosynthetic Gene Cluster Provide Insight into the Biosynthetic Pathway.
  Org. Biomol. Chem., 17 (3), 461-466, doi: 10.1039/c8ob02765j. (2019)

• Kei Sato, Yohei Katsuyama, Kousuke Yokota, Takayoshi Awakawa, Takeaki Tezuka and Yasuo Ohnishi.
  Involvement of β-alkylation machinery and two sets of ketosynthase-chain length factors in the biosynthesis of fogacin polyketides in Actinoplanes missouriensis.
  Chembiochem, 20:1-13, doi:10.1002/cbic.201800640.(2019)

• Linquan Bai, Yasuo Ohnishi and Eung-Soo Kim.
  A3 foresight network on natural products.
  J. Ind. Microbiol. Biotechnol., 46(3-4):313-317, doi:10.1007/s10295-018-2111-8. (2019)

• Fumitaka Kudo, Akimasa Miyanaga and Tadashi Eguchi.
  Structural basis of the nonribosomal codes for nonproteinogenic amino acid selective adenylation enzymes in the biosynthesis of natural products.
  J. Ind. Microbiol. Biotechnol., 46(3-4):515-536, doi:10.1007/s10295-018-2084-7. (2019)

その他の成果

• Tetsuya Yamamoto, Ryo Furuta, Kenji Arakawa and Aiko Teshima.
  Relationship between dispersion-forming capability of poly(4-vinylaniline) colloids and antimicrobial activity.
  COLLOID SURF A PHYSICOCHEM ENG ASP., 596, 124736, doi: 10.1016/j.colsurfa.2020.124736. (2020)

• Shusuke Sato, Fumitaka Kudo, Michel Rohmer and Tadashi Eguchi.
  Characterization of radical SAM adenosylhopane synthase, HpnH, which catalyzes the 5′-deoxyadenosyl radical addition to diploptene in the biosynthesis of C35 bacteriohopanepolyols.
  Angew. Chem. Int. Ed., 59(1), 237-241, doi: 10.1002/anie.201911584. (2020)

• Kei Sakaki, Keita Ohishi, Tetsu Shimizu, Ikki Kobayashi, Naoki Mori, Kenichi Matsuda, Takeo Tomita, Hidenori Watanabe, Kan Tanaka, Tomohisa Kuzuyama and Makoto Nishiyama.
  A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria.
  Nat. Chem. Biol., 16(4), 415-422, doi: 10.1038/s41589-019-0461-9. (2020)

• Fumitaka Kudo, Takeshi Tsunoda, Kaito Yamaguchi, Akimasa Miyanaga and Tadashi Eguchi.
  Stereochemistry in the reaction of the myo-Inositol phosphate synthase ortholog Ari2 during aristeromycin biosynthesis.
  Biochemistry, 58(51), 5112-5116, doi: 10.1021/acs.biochem.9b00981. (2019)

• Kyota Mitsuyama, Takeaki Tezuka and Yasuo Ohnishi.
  Identification and Characterization of a Cell Wall Hydrolase for Sporangiospore Maturation in Actinoplanes missouriensis.
  J. Bacteriol., 201, e00519-19. doi: 10.1128/JB.00519-19. (2019)

• Taro Shiraishi and Tomohisa Kuzuyama.
  Recent advances in the biosynthesis of nucleoside antibiotics.
  J. Antibiot., 72(12), 913-923, doi: 10.1038/s41429-019-0236-2. (2019)

• Eiichiro Kan, Yohei Katsuyama, Jun-ichi Maruyama, Koichi Tamano, Yasuji Koyama and Yasuo Ohnishi.
  Efficient heterologous production of atrochrysone carboxylic acid-related polyketides in an Aspergillus oryzae host with enhanced malonyl-coenzyme A supply.
  J. Gen. Appl. Microbiol., Article ID: 2019.07.001, doi: 10.2323/jgam.2019.07.001. (2019)

• Tetsuya Yamamoto, Kenji Arakawa, Ryo Furuta and Aiko Teshima.
  Influence of the morphology and size of polystyrene synthesized through soap-free emulsion polymerization on antimicrobial activity.
  Materials Today Communication, 20, 100572, doi: 10.1016/j.mtcomm.2019.100572. (2019)

• Tomohiro Kimura, Takeaki Tezuka, Daisuke Nakane, Takayuki Nishizaka, Shin-Ichi Aizawa, and Yasuo Ohnishi.
  Characterization of Zoospore Type IV Pili in Actinoplanes missouriensis.
  J. Bacteriol., 201(14), e00746-18, doi: 10.1128/JB.00746-18. (2019)

• Kei Kudo, Hanae Koiwai, Noritaka Kagaya, Makoto Nishiyama, Tomohisa Kuzuyama, Kazuo Shin-ya* and Haruo Ikeda*.
  Comprehensive Derivatization of Thioviridamides by Heterologous Expression.
  ACS Chem. Biol., 14(6), 1135-1140, doi: 10.1021/acschembio.9b00330. (2019)

• Fumitaka Kudo, Jiahao Zhang, Shusuke Sato, Akane Hirayama and Tadashi Eguchi.
  Functional characterization of 3-aminobenzoic acid adenylation enzyme PctU and UDP-N-acetyl-D-glucosamine: 3-Aminobenzoyl-ACP glycosyltransferase PctL in pactamycin biosynthesis.
  ChemBioChem, 20(19), 2458-2462, 10.1002/cbic.201900239. (2019)

• Eiichiro Kan, Yohei Katsuyama, Jun-ichi Maruyama, Koichi Tamano, Yasuji Koyama and Yasuo Ohnishi.
  Production of the plant polyketide curcumin in Aspergillus oryzae: strengthening malonyl-CoA supply for yield improvement.
  Biosci. Biotechnol. Biochem., 83(7), 1372-1381, doi: 10.1080/09168451.2019.1606699. (2019)

• Jolanta Cieślak, Akimasa Miyanaga, Makoto Takaishi, Fumitaka Kudo and Tadashi Eguchi.
  Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis.
  Acta. Crystallogr F Struct Biol Commun., 75(4), 299-306, doi: 10.1107/S2053230X19002863. (2019)

• Shohei Hayashi, Mai Naka, Kenshin Ikeuchi, Makoto Ohtsuka, Kota Kobayashi, Yasuharu Satoh, Yasushi Ogasawara, Chitose Maruyama, Yoshimitsu Hamano, Tetsuro Ujihara and Tohru Dairi.
  Control mechanism for carbon chain length in polyunsaturated fatty acid synthases.
  Angew. Chem. Int. Ed., doi: 10.1002/anie.201900771. (2019)

• Naoya Toyoda, Tetsuya Yamamoto, Kenji Arakawa and Aiko Teshima.
  Preparation of PVA/polymer colloid nanocomposite hydrogel using PS-PNVA particles.
  Chem. Lett., 48(4), 378-381, doi: 10.1246/cl.181042. (2019)

• Fumihiro Ishikawa, Akimasa Miyanaga, Hinano Kitayama, Shinya Nakamura, Isao Nakanishi, Fumitaka Kudo, Tadashi Eguchi and Genzoh Tanabe.
  An engineered aryl acid adenylation domain with a capacious active site microenvironment.
  Angew. Chem. Int. Ed., doi: 10.1002/anie.201900318. (2019)

• Mamiko Ninomiya, Yoshio Ando, Fumitaka Kudo, Ken Ohmori and Keisuke Suzuki.
  Total Synthesis of Actinorhodin.
  Angew. Chem. Int. Ed., 58(13), 4264-4270, doi: 10.1002/anie.201814172. (2019)

• Ahmed Taha Ayoub, Mohamed Ali Elrefaiy and Kenji Arakawa.
  Computational prediction of the mode of binding of antitumor lankacidin C to tubulin.
  ACS Omega, 4(2), 4461-4471, doi: 10.1021/acsomega.8b03470. (2019)

• Ozaki Taro, Sugiyama Ryousuke, Shimomura Morito, Nishimura Shinichi, Asamizu Shumpei, Katsuyama Yohei, Kakeya Hideaki and Onaka Hiroyasu.
  Identification of the common biosynthetic gene cluster for both antimicrobial streptoaminals and antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines.
  Org. Biomol. Chem., 17, 2370-2378, doi: 10.1039/c8ob02846j. (2019)

• Shohei Hayashi, Yasuharu Satoh, Yasushi Ogasawara, Chitose Maruyama, Yoshimitsu Hamano, Tetsuro Ujihara and Tohru Dairi.
  Control mechanism for cis double bond formation by polyunsaturated fatty acid synthases.
  Angew. Chem. Int. Ed., 58(8), 2326-2330, doi: 10.1002/anie.201812623|. (2019)

• Masaya Kobayashi and Tomohisa Kuzuyama.
  Structural and Mechanistic Insight into Terpene Synthases that Catalyze the Irregular Non-Head-to-Tail Coupling of Prenyl Substrates.
  ChemBioChem, 20(1), 29-33. doi: 10.1002/cbic.201800510. (2019)

• Akimasa Miyanaga.
  Michael additions in polyketide biosynthesis.
  Nat. Prod. Rep., 36(3), 531-547, doi: 10.1039/c8np00071a. (2019)

2018年

本研究プログラムの成果

• Shusuke Sato, Akimasa Miyanaga, Seung-Young Kim, Tomohisa Kuzuyama, Fumitaka Kudo and Tadashi Eguchi.
  Biochemical and structural analysis of FomD that catalyzes the hydrolysis of cytidylyl (S)-2-hydroxypropylphosphonate in fosfomycin biosynthesis.
  Biochemistry, 57(32):4858-4866, doi: 10.1021/acs.biochem.8b00690. (2018)

• Ryota Hagihara, Yohei Katsuyama, Yoshinori Sugai, Hiroyasu Onaka and Yasuo Ohnishi.
  Novel desferrioxamine derivatives synthesized using the secondary metabolism-specific nitrous acid biosynthetic pathway in Streptomyces davawensis.
  J. Antibiot., 71(11):911-919, doi:10.1038/s41429-018-0088-1. (2018)

• Wei Li Thong, Kazuo Shin-ya, Makoto Nishiyama and Tomohisa Kuzuyama.
  Discovery of an Antibacterial Isoindolinone-Containing Tetracyclic Polyketide by Cryptic Gene Activation and Characterization of Its Biosynthetic Gene Cluster.
  ACS Chem. Biol.,13 (9), 2615-2622, doi: 10.1021/acschembio.8b00553. (2018)

• Shusuke Sato, Fumitaka Kudo, Tomohisa Kuzuyama, Friedrich Hammerschmidt and Tadashi Eguchi.
  C-Methylation catalyzed by Fom3, a cobalamin-dependent radical S-adenosyl-L-methionine enzyme in fosfomycin biosynthesis.
  Biochemistry, 57(33):4963-4966, doi:10.1021/acs.biochem.8b00614. (2018)

• Hayama Tsutsumi, Yohei Katsuyama, Miho Izumikawa, Motoki Takagi, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya and Yasuo Ohnishi.
  Unprecedented cyclization catalyzed by a cytochrome P450 in benzastatin biosynthesis.
  J. Am. Chem. Soc., 40 (21), 6631-6639, doi:10.1021/jacs.8b02769. (2018)

• Yusuke Yamauchi, Yosi Nindita, Keisuke Hara, Asako Umeshiro, Yu Yabuuchi, Toshihiro Suzuki, Haruyasu Kinashi and Kenji Arakawa.
  Quinoprotein dehydrogenase functions at the final oxidation step of lankacidin biosynthesis in Streptomyces rochei 7434AN4.
  J. Biosci. Bioeng., 126 (2), 145-152, doi: 10.1016/j.jbiosc.2018.03.006. (2018)

• Kenji Arakawa. Manipulation of metabolic pathway controlled by signaling molecules, inducers of antibiotic production, for genome mining in Streptomyces spp.
  Antonie van Leeuwenhoek., 11 (5), 743-751, doi: 10.1007/s10482-018-1052-6. (2018)

• Danyao Du, Yohei Katsuyama, Kazuo Shin-ya and Yasuo Ohnishi.
  Reconstitution of aType II Polyketide Synthase that Catalyzes Polyene Formation.
  Angew. Chem. Int. Ed., 57, 1954–1957, doi:10.1002/anie.201709636. (2018)

• Yohei Katsuyama, Yukari Sato, Yoshinori Sugai, Yousuke Higashiyama, Miki Senda, Toshiya Senda and Yasuo Ohnishi.
  Crystal structure of the nitrosuccinate lyase CreD in complex with fumarate provides insights into the catalytic mechanism for nitrous acid elimination.
  FEBS J., 285 (8), 1540-1555, doi: 10.1111/febs.14429. (2018)

• Akane Hirayama, Jinmiao Chu, Ena Goto, Fumitaka Kudo and Tadashi Eguchi.
  NAD⁺-dependent dehydrogenase PctP and pyridoxal 5′-phosphate dependent aminotransferase PctC catalyze the first postglycosylation modification of the sugar intermediate in pactamycin biosynthesis.
  ChemBioChem, 19 (2), 126-130, doi: 10.1002/cbic.201700483. (2018)

• Haruka Niikura, Chitose Maruyama, Yasushi Ogasawara, Kazuo Shin-ya, Tohru Dairi and Yoshimitsu Hamano.
  Functional analysis of methyltransferases participating in streptothricin-related antibiotic biosynthesis.
  J. Biosci. Bioeng., 125, 148-154, doi: 10.1016/j.jbiosc.2017.09.004. (2018)

• Si-Sun Choi, Yohei Katsuyama, Linquan Bai, Zixin Deng, Yasuo Ohnishi and Eung-Soo Kim.
  Genome engineering for microbial natural product discovery.
  Curr. Opin. Microbiol., 45, 53-60, doi: 10.1016/j.mib.2018.02.007. (2018)

その他の成果

• Yukie Tanaka, Akari Ikeda, Shigeru Matsukawa, Yuka Imase, Miki Saigyo, Chitose Maruyama, Shigeharu Fujieda and Shu Taira.
  Concurrent mass spectrometric analysis of multiple samples using Py-Tag reagents.
  Int. J. Mass Spectrom., 434, 158-163, doi: 10.1016/j.ijms.2018.09.007. (2018)

• Kenichi Matsuda, Takeo Tomita, Kazuo Shin-ya, Toshiyuki Wakimoto, Tomohisa Kuzuyama and Makoto Nishiyama.
  Discovery of Unprecedented Hydrazine-Forming Machinery in Bacteria.
  J. Am. Chem. Soc., 140 (29), 9083-9086. doi: 10.1021/jacs.8b05354. (2018)

• Akimasa Miyanaga, Risako Ouchi, Fumihiro Ishikawa, Ena Goto, Genzoh Tanabe, Fumitaka Kudo and Tadashi Eguchi.
  Structural basis of protein-protein interactions between a trans-acting acyltransferase and acyl carrier protein in polyketide disorazole biosynthesis.
  J. Am. Chem. Soc., 140(25), 7970-7978, doi:10.1021/jacs.8b04162. (2018)

• Hajime Katano, Mami Maruyama, Yasuhiro Kuroda, Kohei Uematsu, Chitose Maruyama and Yoshimitsu Hamano.
  Partition of amines and lysine oligomers between organic solvent and water under a controlled interfacial potential difference.
  J. Electroanal. Chem., 820, 97-102, doi: 10.1016/j.jelechem.2018.05.001. (2018).

• Akimasa Miyanaga, Fumitaka Kudo and Tadashi Eguchi.
  Protein–protein interactions in polyketide synthase–nonribosomal peptide synthetase hybrid assembly lines.
  Nat. Prod. Rep.., 35(11), 1185-1209, doi:10.1039/c8np00022k. (2018)

• Tetsuya Yamamoto, Kenji Arakawa, Yuya Takahashi and Miho Sumiyoshi.
  Antimicrobial activities of low molecular weight polymers synthesized through soap-free emulsion polymerization.
  Eur. Polym. J., 109, 532-536, doi: 10.1016/j.eurpolymj.2018.10.047. (2018)

• Tetsuya Yamamoto, Kenji Arakawa, Ryo Furuta and Aiko Teshima.
  Antimicrobial activities of polymers synthesized through soap-free emulsion polymerization using a cationic initiator and styrene derivative monomers.
  Chem. Lett., 47(11), 1402-1404, doi: 10.1246/cl.180762. (2018)

• Yoshihiro Mouri, Moon-sun Jang, Kenji Konishi, Aiko Hirata, Takeaki Tezuka and Yasuo Ohnishi.
  Regulation of sporangium formation by the orphan response regulator TcrA in the rare actinomycete Actinoplanes missouriensis.
  Mol. Microbiol., 107(6), 718-733, doi: 10.1111/mmi.13910. (2018)

• Nao Yokoyama, Chihiro Nonaka, Yukari Ohashi, Masaharu Shioda, Takuya Terahata, Wen Chen, Kotomi Sakamoto, Chihiro Maruyama, Takuya Saito, Eiki Yuda, Naoyuki Tanaka, Takashi Fujishiro, Tomohisa Kuzuyama, Kei Asai and Yasuhiro Takahashi.
  Distinct roles for U-type proteins in iron-sulfur cluster biosynthesis revealed by genetic analysis of the Bacillus subtilis sufCDSUB operon.
  Mol. Microbiol., 107 (6), 688-703, doi: 10.1111/mmi.13907. (2018)

• Kyuichi Kawabata, Nobuyuki Baba, Taiken Sakano, Yoshimitsu Hamano, Shu Taira, Akira Tamura, Seigo Baba, Midori Natsume, Takeshi Ishii, Shigeru Murakami and Hajime Ohigashi.
  Functional properties of anti-inflammatory substances from quercetin-treated Bifidobacterium adolescentis.
  Biosci. Biotechnol. Biochem., 82, 689-697, doi: 10.1080/09168451.2017.1401916. (2018)

• Satoru Watanabe, Hiroaki Ozawa, Hiroaki Kato, Kaori Nimura-Matsune, Toshifumi Hirayama, Fumitaka Kudo, Tadashi Eguchi, Katsumi Kakinuma and Hirofumi Yoshikawa.
  Carbon-free production of 2-deoxy-scyllo-inosose (DOI) in cyanobacterium Synechococcus elongatus PCC 7942.
  Biosci. Biotechnol. Biochem., 82 (1), 161-165, doi: 10.1080/09168451.2017.1411777. (2018)

• Kohei Uematsu, Takaaki Ueno, Haruka Kawasaki, Chitose Maruyama, Yoshimitsu Hamano and Hajime Katano.
  Promotion effect of streptothricin on a glucose oxidase enzymatic reaction and its application to a colorimetric assay.
  Anal. Sci., 34, 143-148, doi: 10.2116/analsci.34.143. (2018)

2017年

本研究プログラムの成果

• Akimasa Miyanaga, Ryuichi Takayanagi, Takashi Furuya, Ayano Kawamata, Tomohiro Itagaki, Yoshiharu Iwabuchi, Naoki Kanoh, Fumitaka Kudo and Tadashi Eguchi.
  Substrate recognition by a dual functional P450 monooxygenase GfsF involved in FD-891 biosynthesis.
  Chembiochem, 18 (21), 2179-2187, doi: 10.1002/cbic.201700429. (2017)

• Hiroya Tomita, Yohei Katsuyama, Hiromichi Minami and Yasuo Ohnishi.
  Identification and characterization of a bacterial cytochrome P450 monooxygenase catalyzing the 3-nitration of tyrosine in rufomycin biosynthesis.
  J. Biol. Chem., 292 (38), 15859-15869, doi: 10.1074/jbc.M117.791269. (2017)

• Takeo Tomita, Masaya Kobayashi, Yuma Karita, Yoko Yasuno, Tetsuro Shinada, Makoto Nishiyama and Tomohisa Kuzuyama.
  Structure and mechanism of the monoterpene cyclolavandulyl diphosphate synthase that catalyses consecutive condensation and cyclisation
  Angew. Chem. Int. Ed., 56 (47), 14913-14917, doi: 10.1002/anie.201708474. (2017)

• Su-Hee Cho, Seung-Young Kim, Takeo Tomita, Taro Shiraishi, Jin-Soo Park, Shusuke Sato, Fumitaka Kudo, Tadashi Eguchi, Nobutaka Funa, Makoto Nishiyama and Tomohisa Kuzuyama.
  Fosfomycin biosynthesis via transient cytidylylation of 2-hydroxyethylphosphonate by the bifunctional Fom1 enzyme.
  ACS Chem. Biol., 12 (8), 2209–2215, doi: 10.1021/acschembio.7b00419. (2017)

その他の成果

• Hideo Kawaguchi, Yohei Katsuyama, Danyao Du, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi and Ahikiko Kondo.
  Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli.
  Appl. Microbiol. Biotechnol., 101, 5279-5290, doi: 10.1007/s00253-017-8270-0. (2017)

• Yoshihiro Mouri, Kenji Konishi, Azusa Fujita, Takeaki Tezuka and Yasuo Ohnishi.
  Regulation of sporangium formation by BldD in the rare actinomycete Actinoplanes missouriensis.
  J. Bacteriol., 199, e00840-16., doi: 10.1128/JB.00840-16. (2017)

• Yuji Ishigaki, Genki Akanuma, Minoru Yoshida, Sueharu Horinouchi, Saori Kosono and Yasuo Ohnishi.
  Protein acetylation involved in streptomycin biosynthesis in Streptomyces griseus.
  J. Proteomics, 155, 63-72, doi: 10.1016/j.jprot.2016.12.006. (2017)

• Takaaki Taguchi, Takayoshi Awakawa, Yukitaka Nishihara, Michiho Kawamura, Yasuo Ohnishi and Koji Ichinose.
  Bifunctionality of ActIV as a cyclase-thioesterase revealed by in vitro reconstitution of actinorhodin biosynthesis in Streptomyces coelicolor A3(2).
  Chembiochem, 18, 316-323, doi: 10.1002/cbic.201600589. (2017)

• Kei Kudo, Taro Ozaki, Kazuo Shin-ya, Makoto Nishiyama and Tomohisa Kuzuyama.
  Biosynthetic origin of the hydroxamic acid moiety of trichostatin A: identification of unprecedented enzymatic machinery involved in hydroxylamine transfer.
  J. Am. Chem. Soc., 139(20), 6799-6802, doi: 10.1021/jacs.7b02071. (2017)

• Takeo Tomita, Lulu Yin, Shugo Nakamura, Saori Kosono, Tomohisa Kuzuyama and Makoto Nishiyama.
  Crystal structure of the 2-iminoglutarate-bound complex of glutamate dehydrogenase from Corynebacterium glutamicum.
  FEBS Lett., 591 (11), 1611-1622, doi: 10.1002/1873-3468.12667. (2017)

• Takeo Tomita, Seung-Young Kim, Kazuya Teramoto, Ayuko Meguro, Taro Ozaki, Ayako Yoshida, Yudai Motoyoshi, Naoki Mori, Ken Ishigami, Hidenori Watanabe, Makoto Nishiyama and Tomohisa Kuzuyama.
  Structural insights into the CotB2-catalyzed cyclization of geranylgeranyl diphosphate to the diterpene cyclooctat-9-en-7-ol.
  ACS Chem. Biol., 12(6), 1621-1628, doi: 10.1021/acschembio.7b00154. (2017)

• Tomohisa Kuzuyama.
  Biosynthetic studies on terpenoids produced by Streptomyces.
  J. Antibiot., 70(7), 811-818. doi: 10.1038/ja.2017.12. (2017)

• Ayako Nara, Takuya Hashimoto, Mamoru Komatsu, Makoto Nishiyama, Tomohisa Kuzuyama and Haruo Ikeda.
  Characterization of bafilomycin biosynthesis in Kitasatospora setae KM-6054 and comparative analysis of gene clusters in Actinomycetales microorganisms.
  J. Antibiot., 70 (5), 616-624, doi: 10.1038/ja.2017.33. (2017)

• Shusuke Sato, Fumitaka Kudo, Seung-Young Kim, Tomohisa Kuzuyama and Tadashi Eguchi.
  Methylcobalamin-dependent radical SAM C-methyltransferase Fom3 recognizes cytidylyl-2-hydroxyethylphosphonate and catalyzes the nonstereoselective C-methylation in fosfomycin biosynthesis.
  Biochemistry, 56 (28), 3519-3522, doi: 10.1021/acs.biochem.7b00472. (2017)

• Taichi Chisuga, Akimasa Miyanaga, Fumitaka Kudo and Tadashi Eguchi.
  Structural analysis of the dual function thioesterase SAV606 unravels the mechanism of Michael addition of glycine to an α,β-unsaturated thioester.
  J. Biol. Chem., 292 (26), 10926-10937, doi: 10.1074/jbc.M117.792549. (2017)

• Jolanta Cieślak, Akimasa Miyanaga, Ryoma Takaku, Makoto Takaishi, Keita Amagai, Fumitaka Kudo and Tadashi Eguchi.
  Biochemical characterization and structural insight into aliphatic β-amino acid adenylation enzymes IdnL1 and CmiS6.
  Proteins, 85 (7), 1238-1247, DOI: 10.1002/prot.25284. (2017)

• Fumitaka Kudo, Takahiro Tokumitsu and Tadashi Eguchi.
  Substrate specificity of radical S-adenosyl-L-methionine dehydratase AprD4 and its partner reductase AprD3 in the C3′-deoxygenation of aminoglycoside antibiotics.
  J. Antibiot., 70 (4), 423-428, DOI: 10.1038/ja.2016.110. (2017)

• Satomi Fujita, Su-Hee Cho, Ayako Yoshida, Fumihito Hasebe, Takeo Tomita, Tomohisa Kuzuyama and Makoto Nishiyama.
  Crystal structure of LysK, an enzyme catalyzing the last step of lysine biosynthesis in Thermus thermophilus, in complex with lysine: Insight into the mechanism for recognition of the amino-group carrier protein, LysW.
  Biochem. Biophys. Res. Commun., 491 (2), 409-415, doi: 10.1016/j.bbrc.2017.07.088. (2017)

• Rikiya Kamei, Takashi Fujimura, Miki Matsuda, Kotaro Kakihara, Noriko Hirakawa, Kenji Baba, Kazuhisa Ono, Kenji Arakawa and Seiji Kawamoto.
  A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions.
  Biochem. Biophys. Res. Commun., 483, 674-679, doi: 10.1016/j.bbrc.2016.12.083. (2017)

• Natsumi Tsujita, Hiroyuki Kuwahara, Hiroki Koyama, Noriyuki Yanaka, Kenji Arakawa and Hisato Kuniyoshi.
  Molecular characterization of aspartylglucosaminidase, a lysosomal hydrolase upregulated during strobilation in the moon jellyfish, Aurelia aurita.
  Biosci. Biotechnol. Biochem., 81, 938-950, doi: 10.1080/09168451.2017.1285686 (2017)

• Hajime Katano, Yasuhiro Kuroda, Shu Taira, Chitose Maruyama and Yoshimitsu Hamano.
  Colorimetric microtiter plate assay of polycationic aminoglycoside antibiotics in culture broth using amaranth.
  Anal. Sci., 33, 499-504, doi: 10.2116/analsci.33.499. (2017)

• Kazunori Ushimaru, Chitose Maruyama, Yoshimitsu Hamano and Hajime Katano.
  Antimicrobial activity of ε poly L lysine after forming a water-insoluble complex with an anionic surfactant.
  Biomacromolecules, 18, 1387-1392, doi: 10.1021/acs.biomac.7b00109. (2017)

• Taro Ozaki, Kona Yamashita, Yuki Goto, Morito Shimomura, Shohei Hayashi, Shumpei Asamizu, Yoshinori Sugai, Haruo Ikeda, Hiroaki Suga and Hiroyasu Onaka.
  Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo.
  Nat. Commun., 8, 14207, doi: 10.1038/ncomms14207. (2017)

2016年（7月以降）

その他の成果

• Mai Tsujimoto, Ayako Yoshida, Tetsu Shimizu, Takeo Tomita, Yasuo Ohnishi, Tomohisa Kuzuyama and Makoto Nishiyama.
  Aspartate kinase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis in Streptomyces murayamaensis.
  Biosci. Biotechnol. Biochem., 80 (11), 2255-2263. doi: 10.1080/09168451.2016.1214534. (2016)

• Moon-Sun Jang, Yoshihiro Mouri, Kaoru Uchida, Shin-Ichi Aizawa, Masayuki Hayakawa, Nobuyuki Fujita, Takeaki Tezuka and Yasuo Ohnishi.
  Genetic and transcriptional analyses of the flagellar gene cluster in Actinoplanes missouriensis.
  J. Bacteriol., 198 (16), 2219-2227. doi: 10.1128/JB.00306-16. (2016)

• Akihiro Ninomiya, Yohei Katsuyama, Takefumi Kuranaga, Masayuki Miyazaki, Yuichi Nogi, Shigeru Okada, Toshiyuki Wakimoto, Yasuo Ohnishi, Shigeki Matsunaga and Kentaro Takada.
  Biosynthetic gene gluster for surugamide a encompasses an unrelated decapeptide, surugamide F.
  Chembiochem, 17 (18), 1709-1712. doi: 10.1002/cbic.201600350. (2016)

• Danyao Du, Yohei Katsuyama, Hiroyasu Onaka, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya and Yasuo Ohnishi.
  Production of a novel amide-containing polyene by activating a cryptic biosynthetic gene cluster in Streptomyces sp. MSC090213JE08.
  Chembiochem, 17 (15), 1464-1471. doi: 10.1002/cbic.201600167. (2016)

• Takuya Hashimoto and Tomohisa Kuzuyama.
  Mechanistic insights into Diels-Alder reactions in natural product biosynthesis.
  Curr. Opin. Chem. Biol., 35, 117-123. doi: 10.1016/j.cbpa.2016.09.015. (2016)

• Taro Shiraishi, Noboru Hiro, Masayuki Igarashi, Makoto Nishiyama and Tomohisa Kuzuyama.
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